Ganoderma lucidum ethanolic extract for the treatment of androgenic alopecia in rats with testosterone-induced Baldness.


  • Poonam C. Rahangdale Department of Pharmacology, Vidyabharti Collage of Pharmacy Amravati, Mahar astra,444602, India
  • Anjali M. Wankhade Department of Pharmacology, Vidyabharti Collage of Pharmacy Amravati, Mahar astra,444602, India



Androgenic alopecia, Hair growth, Ethanolic extract of Ganoderma lucidum, follicular density, testosterone, Minoxidil 2%


Many people worldwide suffer from alopecia. The current study's objective was to investigate the hair growth-promoting properties of a preparation containing an ethanolic extract of Ganoderma lucidum and its primary components on hair regeneration using a model of testosterone-induced alopecia. The five groups of rats tested were: negative control group (n = 6); positive control group (n = 6); testosterone plus minoxidil 2% (n = 6); testosterone and ethanolic extract of Ganoderma lucidum extract (10 mg/kg) (n = 6); and testosterone and ethanolic extract of Ganoderma lucidum extract (15 mg/kg). day-inducing substance to all groups except the negative control group, subcutaneous testosterone (1 mg/kg Sc) was administered for 21 straight days. Prior to 1 hour, the standard group received topical application of 2% minoxidil, EEGL at 10 mg/kg and 15 mg/kg, respectively. Following 21 days, a photograph was taken to document the variations in hair development between each group. After the study was finished, all of the animals were slaughtered, and the area was subsequently examined for various characteristics to gauge hair development. Anagen telogen ratio and the number of follicles per square millimetre were measured and reported. Anagen/telogen (A/T) ratio, follicular density, and hair length were all higher in the standard. EEGL 10mg/kg and EEGL 15mg/kg treatment groups were evaluated in comparison to a positive control group. The anagen/telogen ratio of the hair follicles as well as their quantity and shape both increased in EEGL. Through the return of normal parameters, the EEGL was able to promote hair growth. Compared to EEGL10mg/kg, EEGL15mg/kg produced more favourable outcomes. Its outcomes were equivalent to those of Minoxidil 2% solution, a commonly recommended drug used to encourage hair growth in persons with androgenetic alopecia.


Itankar PR, Thakre PT, Murkute AV, Tauqeer MO. Effect of medicated oil of Martynia annua leaves and fruits on testosterone induced alopecia in mice. Asian J Pharm Clin Res. 2013;6(5):49-52..

Sperling LC. Hair and systematic disease. Dermatologic clinics. 2001 Oct 1;19(4):711-26.

França K, Rodrigues TS, Ledon J, Savas J, Chacon A. Comprehensive overview and treatment update on hair loss.

Devjani S, Ezemma O, Kelley KJ, Stratton E, Senna M. Androgenetic Alopecia: Therapy Update. Drugs. 2023 May 11:1-5

Olsen EA. Androgenetic alopecia. Disorders of hair growth: diagnosis and treatment. 1994.

Paus R, Cotsarelis G. The biology of hair follicles. New England journal of medicine. 1999 Aug 12;341(7):491-7.

Randall VA, Thornton MJ, Hamada K, Redfern CP, Nutbrown M, Ebling FJ, Messenger AG. Androgens and the Hair Follicle: Cultured Human Dermal Papilla Cells as a Model System a. Annals of the New York Academy of Sciences. 1991 Dec;642(1):355-75.

Whiting DA. Diagnostic and predictive value of horizontal sections of scalp biopsy specimens in male pattern androgenetic alopecia. Journal of the American Academy of Dermatology. 1993 May 1;28(5):755-63.

Price VH. Treatment of hair loss. New England Journal of Medicine. 1999 Sep 23;341(13):964-73.

Roy RK, Thakur M, Dixit VK. Development and evaluation of polyherbal formulation for hair growth–promoting activity. Journal of cosmetic dermatology. 2007 Jun;6(2):108-12..

Wachtel-Galor S, Yuen J, Buswell JA, Benzie IF. Ganoderma lucidum (Lingzhi or Reishi). Herbal Medicine: Biomolecular and Clinical Aspects. 2nd edition. 2011.

Amdekar S. Ganoderma lucidum (Reishi): source of pharmacologically active compounds. Curr Sci. 2016 Sep 25;111(6):976-8.

Sanodiya BS, Thakur GS, Baghel RK, Prasad GB, Bisen PS. Ganoderma lucidum: a potent pharmacological macrofungus. Current pharmaceutical biotechnology. 2009 Dec 1;10(8):717-42.

Ma B, Ren W, Zhou Y, Ma J, Ruan Y, Wen CN. Triterpenoids from the spores of Ganoderma lucidum. North American journal of medical sciences. 2011 Nov;3(11):495.

Ugwu MN, Eteng MU, Omang WA, Eno MA. Effect of Vernonia amygdalina (VA) on oxidative stress status of benign prostatic hyperplasia induced-wistar rats. Asian J Res Med Pharm Sci. 2018;4(3):1-1.

Kaushik R, Gupta D, Yadav R. Alopecia: herbal remedies. International Journal of Pharmaceutical Sciences and Research. 2011 Jul 1;2(7):1631.

Stallard N, Whitehead A, Indans I. Statistical evaluation of an acute dermal toxicity test using the dermal fixed dose procedure. Human & experimental toxicology. 2004 Aug;23(8):405-12.

Smina TP, Nitha B, Devasagayam TP, Janardhanan KK. Ganoderma lucidum total triterpenes induce apoptosis in MCF-7 cells and attenuate DMBA induced mammary and skin carcinomas in experimental animals. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2017 Jan 1;813:45-51.

Noubarani M, Rostamkhani H, Erfan M, Kamalinejad M, Eskandari MR, Babaeian M, Salamzadeh J. Effect of Adiantum capillus veneris linn on an animal model of testosterone-induced hair loss. Iranian Journal of Pharmaceutical Research: IJPR. 2014;13(Suppl):113.

National Research Council. Education and training in the care and use of laboratory animals: a guide for developing institutional programs.

Nischal U, Nischal KC, Khopkar U. Techniques of skin biopsy and practical considerations. Journal of cutaneous and aesthetic surgery. 2008 Jul;1(2):107.

Elston DM, Stratman EJ, Miller SJ. Skin biopsy: Biopsy issues in specific diseases. Journal of the American Academy of Dermatology. 2016 Jan 1;74(1):1-6.

Dhariwala MY, Ravikumar P. An overview of herbal alternatives in androgenetic alopecia. Journal of cosmetic dermatology. 2019 Aug;18(4):966-75.

Angad Patole, Rajendra Ganjiwale, Protective Effect of Ellagic Acid on Testosterone-induced Alopecia in Rats. Asian Journal of Biological Sciences.2020.158.167

Hajhashemi V, Rajabi P, Mardani M. Beneficial effects of pumpkin seed oil as a topical hair growth promoting agent in a mice model. Avicenna journal of phytomedicine. 2019 Nov;9(6):499.

Trüeb RM. Molecular mechanisms of androgenetic alopecia. Experimental gerontology. 2002 Aug 9;37(8-9):981-90.

Liu J, Kurashiki K, Shimizu K, Kondo R. 5α-Reductase inhibitory effect of triterpenoids isolated from Ganoderma lucidum. Biological and Pharmaceutical Bulletin. 2006;29(2):392-5.




How to Cite

Rahangdale, P., & Wankhade, A. M. (2023). Ganoderma lucidum ethanolic extract for the treatment of androgenic alopecia in rats with testosterone-induced Baldness. Medical and Pharmaceutical Journal, 2(2), 107–120.