Histological Investigation to Verify the Protective Advantages of Carissa Spinarum Leaves Extract in Zebrafish (Danio rerio)
DOI:
https://doi.org/10.55940/medphar202499Keywords:
Multiple organ protective effect, Zebrafish, Danio rerio, Carissa spinarum extract, Acute toxicity studiesAbstract
Background: Carissa spinarum, a traditional medicinal plant, is known for its therapeutic properties, including hepatoprotective and antioxidant effects.
Objective: A study was conducted to determine whether the herb Carissa spinarum leaf extract has a protective impact on certain organs.
Methods: Adult wild strain zebrafish (Danio rerio) were used in an acute toxicity study, and a dose of 5 ppm (parts per million) of carbon tetra chloride was used to cause multiple organ damage. An aqua-alcoholic extract made from the shadow-dried leaves of the plant Carissa spinarum was administered to the animals in seven groups of ten each at concentrations of 0.5 ppm, 1 ppm, and 2 ppm. Standard protective medication utilized was silymarin at a dose of 50 ppm.
Results: Plant extract was administered to the groups at levels of 0.5 ppm, 1 ppm, and 2 ppm; in all of these groups, the multiple organ protective effect was seen. At the highest dose of 2 ppm, the greatest protective effect was observed. The tissue of the skeletal muscles showed no effects.
Conclusion: : The goal of the entire experiment was to determine whether an extract made from Carissa spinarum plant leaves had any protective effects on organs. The kidney, gut, and gills of zebrafish recovered more quickly after the plant extract was administered, demonstrating that it also had regenerative qualities. Lastly, the results showed that the extract made from the leaves of the Carissa spinarum plant had several organ protective effects in addition to hepatoprotective effects.
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Copyright (c) 2025 Shiwank Rana, Nitin Sharma, Sunil Dutt, Rajendra Damu Patil, Deepika Rana
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